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Increased sIL-15R α levels in RA synovial fluids. The graphic represents median and interquartile range. Mann-Whitney test shows a significant difference, P = 0.0025, between OA and RA groups.

Journal: Arthritis

Article Title: Proinflammatory Soluble Interleukin-15 Receptor Alpha Is Increased in Rheumatoid Arthritis

doi: 10.1155/2012/943156

Figure Lengend Snippet: Increased sIL-15R α levels in RA synovial fluids. The graphic represents median and interquartile range. Mann-Whitney test shows a significant difference, P = 0.0025, between OA and RA groups.

Article Snippet: Therefore, we detected IL-15R α using an anti-IL-15R α antibody (AF247, R&D) as a detection antibody as previously described [ ].

Techniques: MANN-WHITNEY

Western blot analysis of IL-15R α in synovial fluids from RA patients. Proteins from synovial fluids from different patients (lanes 1–5) were separated on 12.5% SDS-PAGE and transfer to nitrocellulose for western blotting. The western blot was probed with anti-IL-15R α antibody and development with anti-goat-HRP. Representative western blot is shown.

Journal: Arthritis

Article Title: Proinflammatory Soluble Interleukin-15 Receptor Alpha Is Increased in Rheumatoid Arthritis

doi: 10.1155/2012/943156

Figure Lengend Snippet: Western blot analysis of IL-15R α in synovial fluids from RA patients. Proteins from synovial fluids from different patients (lanes 1–5) were separated on 12.5% SDS-PAGE and transfer to nitrocellulose for western blotting. The western blot was probed with anti-IL-15R α antibody and development with anti-goat-HRP. Representative western blot is shown.

Article Snippet: Therefore, we detected IL-15R α using an anti-IL-15R α antibody (AF247, R&D) as a detection antibody as previously described [ ].

Techniques: Western Blot, SDS Page

Effect of IL-15R α on IL-6 secretion in synovial cells. Treatment with 250 ng/mL of IL-15R α significantly upregulated IL-6 expression. Each bar represents the mean and SD of two determinations. ** = P < 0.001.

Journal: Arthritis

Article Title: Proinflammatory Soluble Interleukin-15 Receptor Alpha Is Increased in Rheumatoid Arthritis

doi: 10.1155/2012/943156

Figure Lengend Snippet: Effect of IL-15R α on IL-6 secretion in synovial cells. Treatment with 250 ng/mL of IL-15R α significantly upregulated IL-6 expression. Each bar represents the mean and SD of two determinations. ** = P < 0.001.

Article Snippet: Therefore, we detected IL-15R α using an anti-IL-15R α antibody (AF247, R&D) as a detection antibody as previously described [ ].

Techniques: Expressing